Fig 1: LPS-Nb36 enhances cytotoxicity of CD8+ T cells activated by DC/tumor fusion vaccine.A The CD8+ T cells were co-incubated with PKH26-prestained HepG2, A549 or MGC-803 cells with DC/tumor fusion vaccine at E/T ratio 1:1,5:1,10:1 or 20:1, with LPS, LPS-Nb36, Nb36, anti-CTLA-4 mAb or LPS-CTLA-4 mAb for 6 h. propidium iodide(Pl)was used for lysed cell staining. The ratios of PHK26+PI+cell were measured by flow cytometry. n = 3, *P < 0.05, **P < 0.01. B After co-culturing with CAFs at a 20:1 ratio for 6 h, the IFN-γ(intracellular) and CD107a expression of effector cells were measured by flow cytometry. n = 3, ***P < 0.001.